pe cytokeratin  (Novus Biologicals)

Journal: Disease Models & Mechanisms

Article Title: Murine cell lines with defined mutations model different histological subtypes of epithelial ovarian cancer

doi: 10.1242/dmm.052177

Figure Lengend Snippet: Cell lines reproduce different ovarian cancer histotypes and have different chemokine secretion profiles. (A) Syngeneic mice were injected IP with the specified cell lines. Tumors obtained at necropsy were fixed in formalin and paraffin embedded. Four-micron sections were used for HE staining (top row), dual CD3 (brown)/CD20 (red) IHC (middle row) and chip cytometry with four fluorescent markers (WT1, red; HNF1β, blue; pan-cytokeratin, white; vimentin, green) (bottom row). Scale bars: 50 µm. (B) Heterogeneity of secreted cytokine and chemokines across 29 murine cell lines. Murine IP-10 (CXCL10) and eotaxin were measured in cell culture supernatants by Meso Scale Discovery. MCP-1 was measured by enzyme-linked immunosorbent assay. Values represent average concentration (in pg/ml) of two technical replicates. Key is shown on the right. Additional measurements are shown in .

Article Snippet: The following antibodies were used to stain the prepared tissue chips: anti-HNF1B polyclonal antibody (Proteintech, 12533-1-AP, 1:1500) with PE-donkey-anti-rabbit IgG (minimal x-reactivity; BioLegend, 406421, 1:300) as the secondary antibody, BUV395 mouse anti-human MUC1 (BD Biosciences, CD227, 1:300), Alexa Fluor ® 488 anti-vimentin (BioLegend, 677809, 1:600), Alexa Fluor ® 488 anti-WT1 (Abcam, ab202635, 1:200), PE-cytokeratin, pan antibody (Novus, C-11, 1:1500) and eFluorTM 570 anti-alpha-smooth muscle actin (eBioscience, 1A4, 1:600).

Techniques: Injection, Staining, Chip Cytometry, Cell Culture, Enzyme-linked Immunosorbent Assay, Concentration Assay

Journal: Brazilian Journal of Medical and Biological Research

Article Title: 1α,25-Dihydroxyvitamin D3 accelerates skin wound re-epithelialization by promoting epidermal stem cell proliferation and differentiation through PI3K activation: an in vitro and in vivo study

doi: 10.1590/1414-431X2025e14121

Figure Lengend Snippet: Vitamin D3 promoted the differentiation of epidermal stem cells (EpSCs) in wounds. Representative immunofluorescence-stained sections for co-expression of ( A ) K10 and K14 in wound tissues (scale bars: 100 μm) and the expression of ( B ) K10 in wound epidermis (scale bar: 10 μm) on the 5th day after wounding, and quantitative data (right panels) (n=3/group). Data are reported as means±SD. *P<0.05, **P<0.01, and ***P<0.001 compared with control group; NS: non-significant (ANOVA).

Article Snippet: The antibodies used were CD49f-BV421 (1:200, BioLegend, 313624, USA), CD71-PE (1:200, Invitrogen, 2024832, USA), K15-PE (1:200, Santa Cruz Biotechnology, sc-47679, USA), K14-AF488 (1:200, Santa Cruz Biotechnology, sc-53253), and K10-PE (1:200, Santa Cruz Biotechnology, sc-53251).

Techniques: Immunofluorescence, Staining, Expressing, Control

Journal: Brazilian Journal of Medical and Biological Research

Article Title: 1α,25-Dihydroxyvitamin D3 accelerates skin wound re-epithelialization by promoting epidermal stem cell proliferation and differentiation through PI3K activation: an in vitro and in vivo study

doi: 10.1590/1414-431X2025e14121

Figure Lengend Snippet: Vitamin D3 promoted proliferation and differentiation of epidermal stem cells (EpSCs) in vitro . A , The proliferation of skin epidermal stem cells from C57BL/6 mice treated with different doses of vitamin D3 (0, 1, 10, 50 nM) for 48 h was detected by CCK8 (n=3/group). K15+ ( B ), CD49f +/CD71- ( C ), K14+ ( D ), and K10+ ( E ) cells were detected by flow cytometry after the EpSCs were treated with different doses of vitamin D (0, 1, 10, 50 nM) for 48 h, and quantitative data (right panel) (n=3/group). Data are reported as means±SD. *P<0.05 and **P<0.01 compared with control group (ANOVA).

Article Snippet: The antibodies used were CD49f-BV421 (1:200, BioLegend, 313624, USA), CD71-PE (1:200, Invitrogen, 2024832, USA), K15-PE (1:200, Santa Cruz Biotechnology, sc-47679, USA), K14-AF488 (1:200, Santa Cruz Biotechnology, sc-53253), and K10-PE (1:200, Santa Cruz Biotechnology, sc-53251).

Techniques: In Vitro, Flow Cytometry, Control

Journal: Brazilian Journal of Medical and Biological Research

Article Title: 1α,25-Dihydroxyvitamin D3 accelerates skin wound re-epithelialization by promoting epidermal stem cell proliferation and differentiation through PI3K activation: an in vitro and in vivo study

doi: 10.1590/1414-431X2025e14121

Figure Lengend Snippet: Vitamin D enhanced the proliferation and differentiation of epidermal stem cells by activating PI3K signaling pathway. A , Representative immunoblotting for the expression of p-PI3K and t-PI3K (n=3/group). B , The proliferation of skin epidermal stem cells treated with vitamin D or LY294002 for 48 h was detected by CCK8 (n=3/group). C , Representative immunofluorescence-stained sections (scale bars: 10 μm) for co-expression of K10 and K14 in wound epidermis tissues at day five after wounding, and quantitative data (right panel) (n=3/group). Data are reported as means±SD. ***P<0.001 compared with control groups (ANOVA).

Article Snippet: The antibodies used were CD49f-BV421 (1:200, BioLegend, 313624, USA), CD71-PE (1:200, Invitrogen, 2024832, USA), K15-PE (1:200, Santa Cruz Biotechnology, sc-47679, USA), K14-AF488 (1:200, Santa Cruz Biotechnology, sc-53253), and K10-PE (1:200, Santa Cruz Biotechnology, sc-53251).

Techniques: Western Blot, Expressing, Immunofluorescence, Staining, Control